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Part:BBa_K914007:Design

Designed by: Denis Samuylov   Group: iGEM12_Paris_Bettencourt   (2012-09-20)

Meganuclease I-SceI controlled by pBad


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The system is leaky and meganuclease cut RS with high efficiency even without pBad induction.

Sequence

The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG)


Source

Promoter pBad and RBS was taken from an iGEM distribution plate (BBa_I13453 and BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).

References